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Large-scale protein identification using mass spectrometry.

Dayin Lin1, David L Tabb, John R Yates

  • 1Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd., La Jolla, CA 92037, USA.

Biochimica Et Biophysica Acta
|March 15, 2003
PubMed
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Genomics advancements fuel proteomics, enabling high-throughput protein identification through mass spectrometry. This review explores mass spectrometry methods crucial for large-scale protein studies in cells and complexes.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Bioinformatics

Background:

  • Genomics has established a robust biological information infrastructure.
  • This success has spurred rapid growth in proteomics, the study of proteins.
  • Proteomics investigates proteins within complexes, organelles, and cells.

Purpose of the Study:

  • To review mass spectrometry methods for large-scale protein identification.
  • To highlight applications of mass spectrometry in proteomics.
  • To emphasize the importance of high-throughput protein identification.

Main Methods:

  • Mass spectrometry (MS) is a key technology for protein identification.
  • The review focuses on MS methods applicable to large-scale proteomics.

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  • Discussion includes techniques for protein identification, mapping, quantification, and interaction studies.

    • Main Results:

    • Mass spectrometry enables high-throughput protein identification.
    • It is essential for studying dynamic changes in protein expression and interactions.
    • MS facilitates comprehensive proteome mapping and differential expression analysis.

    Conclusions:

    • Mass spectrometry is a fundamental tool for modern proteomics.
    • High-throughput protein identification is critical for understanding cellular processes.
    • The review provides insights into MS methods and their applications in large-scale protein analysis.