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Binding of antibromelain monomeric Fab' improves the stability of stem bromelain against inactivation
Pawan Gupta1, Rizwan H Khan, M Saleemuddin
1Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Uttar Pradesh, India.
Biochimica Et Biophysica Acta
|March 15, 2003
Abstract:
Antienzyme polyclonal antibodies against stem bromelain were raised in male albino rabbits and the Fab' monomers isolated from the IgG of the immune sera. Incubation of bromelain with the Fab' resulted in binding and gel filtration of the resulting complex suggested a 1:1 stoichiometry. Complexing with the Fab' resulted in significant stabilization of bromelain against thermal inactivation and alkaline pH.
