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Related Experiment Videos

Novel bacterial ratio for predicting faecal age.

J Nieman1, G M Brion

  • 1Dept of Civil Engineering, University of Kentucky, 161 Raymond Bldg., Lexington, KY 40506-0281, USA.

Water Science and Technology : a Journal of the International Association on Water Pollution Research
|March 18, 2003
PubMed
Summary

The ratio of atypical colonies to total coliforms in water increases over time, indicating changes in bacterial populations. This finding is crucial for assessing water quality and potential contamination sources.

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Area of Science:

  • Environmental microbiology
  • Water quality assessment
  • Bacteriology

Background:

  • Total coliform testing is a standard method for water quality monitoring.
  • Understanding shifts in bacterial populations is vital for accurate water safety evaluations.
  • The ratio of atypical colonies (AC) to total coliform colonies (TC) may offer new insights.

Purpose of the Study:

  • To investigate the utility of the AC/TC ratio in assessing changes in indicator bacterial populations over time.
  • To correlate the AC/TC ratio with water quality parameters and potential contamination sources.
  • To extend ongoing research on coliform testing using m-Endo broth media.

Main Methods:

  • Laboratory-based survival studies using filtered river water spiked with coliforms.

Related Experiment Videos

  • Analysis of two years of water quality data from the Kentucky River.
  • Monitoring the AC/TC ratio in samples held over time in laboratory conditions.

    • Main Results:

    • The AC/TC ratio significantly increased over time in laboratory survival studies, rising from <1 to >200 within 4 days.
    • Analysis of Kentucky River data confirmed this trend, with higher AC/TC ratios during low-flow months (average 27.58) compared to high-flow months (average 3.37).
    • The AC/TC ratio during low-flow periods aligned with values associated with animal-impacted urban runoff, while high-flow ratios were closer to raw human sewage.

    Conclusions:

    • The AC/TC ratio is a sensitive indicator of bacterial population shifts in river water over time.
    • Changes in the AC/TC ratio can help differentiate between contamination sources, such as human sewage versus urban runoff.
    • This ratio provides a valuable tool for enhanced water quality monitoring and risk assessment.