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Published on: December 15, 2015
The optimization of HPLC-UV conditions for use with FTIR detection in the analysis of B vitamins
1Department of Chemistry, University of Rhode Island, Kingston, RI, 02881, USA.
Abstract:
The water-soluble vitamins thiamine (B(1)), riboflavin (B(2)), pantothenic acid (B(5)), and pyridoxine (B(6)) are separated by high-performance liquid chromatography. The mobile phase, column temperature, and flow rate are optimized so that the chromatograph can be used with a Fourier-transform infrared (FTIR) detector. Reproducibility, linearity, and detection limits are evaluated for method validation. Finally, this method is successfully transferred to liquid chromatography-FTIR with a standard mixture.
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