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Anti-platelet fraction from Galega officinalis L. inhibits platelet aggregation
Atanas Todorov Atanasov1, Bojidar Tchorbanov
1Department of Biophysics, Faculty of Medicine, Thracian University, Stara Zagora 6000, Bulgaria. atanastod@abv.bg
Abstract:
A fraction from crude extract of Galega officinalis L. was purified by gel filtration on Sephadex G-25, Sepharose 4B, and ion-exchange chromatography on diethylaminoethyl (DEAE)-cellulose. The fraction with molecular weight 100-140 kDa appears to have a polysaccharide nature, including protein. The fraction inhibits platelet aggregation initiated by 25 microM adenosine 5'-diphosphate (ADP), 100 microg/ml collagen, and 0.8 U/ml thrombin with the 50% inhibiting concentration (IC(50)) being 11.2 microg/ml for ADP, and the IC(100) being 15.1 microg/ml for collagen and IC(100) 19.6 microg/ml for thrombin.