Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Exploring the Penicillium marneffei genome.

Kwok-Yung Yuen1, Géraldine Pascal, Samson S Y Wong

  • 1HKU-Pasteur Research Centre, 8 Sassoon Road, Hong Kong. kyyuen@hkucc.hku.hk

Archives of Microbiology
|March 18, 2003
PubMed
Summary

Genomic analysis of Penicillium marneffei, a fungus infecting AIDS patients, revealed its genome size and key genes. This study provides insights into fungal dimorphism and potential drug resistance mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

16S rRNA sequence dataset for the identification of the French hoverflies (Diptera, Syrphidae).

Biodiversity data journal·2026
Same author

Unraveling myoepithelial cell plasticity in breast cancer: a transcriptomic approach.

Journal of histotechnology·2026
Same author

The fecal microbiota of lactating Holstein cows: A meta-analysis highlighting key microbial profiles and methodological challenges.

Journal of dairy science·2026
Same author

Scientists' Warning to Humanity: The Need to Begin Teaching Critical and Systems Thinking Early in Life.

Microbial biotechnology·2025
Same author

Metabolic Origin, Role and Fate of the Denaturant Guanidine.

Microbial biotechnology·2025
Same author

The Diversity of Oligosaccharide Composition in Rabbit Milk and Its Association with the Development of the Cecal Microbiota and the Survival of Young Rabbits.

The Journal of nutrition·2025

Area of Science:

  • Mycology
  • Genomics
  • Infectious Diseases

Background:

  • Penicillium marneffei is a dimorphic fungus endemic to Southeast Asia.
  • It causes opportunistic infections, particularly in 10% of AIDS patients in the region.
  • Challenges in studying its thermal dimorphism and host interactions stem from its asexual reproduction and infectious nature.

Purpose of the Study:

  • To conduct genomic analysis of Penicillium marneffei to understand its biology.
  • To identify genes related to thermal dimorphism, host-microbe interactions, and drug resistance.
  • To investigate the potential for a sexual stage in Penicillium marneffei.

Main Methods:

  • Pulsed-field gel electrophoresis (PFGE) was used to analyze genomic DNA.
  • Telomeric fingerprinting was employed to estimate chromosome number and size.

Related Experiment Videos

  • Random sequence tags (RSTs) were generated for genome exploration and gene identification.

    • Main Results:

    • The genome size of Penicillium marneffei was estimated to be 17.8–26.2 Mb with a G+C content of 48.8 mol%.
    • Analysis of 2303 RSTs revealed genes involved in information transfer, metabolism, and compartmentalization.
    • Several multi-drug-resistance protein genes and fluconazole-resistance gene homologues were identified.
    • Genes encoding pheromone homologues and ankyrin repeat proteins suggest a potential sexual stage.

    Conclusions:

    • Genomic analysis provides crucial insights into Penicillium marneffei.
    • The identified genes offer targets for understanding fungal dimorphism and developing antifungal strategies.
    • Evidence suggests the presence of a sexual stage, potentially existing in the environment.