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Preparative-scale isoelectric trapping enantiomer separations
1Department of Chemistry, MS 3255, Texas A&M University, PO Box 30012, College Station, TX 77842-3012, USA.
Journal of Chromatography. A
|March 19, 2003
Summary
A modified Gradiflow BF200 isoelectric trapping (IET) unit enables preparative-scale enantiomer separations using hydroxypropyl beta-cyclodextrin. This enhanced IET device achieves significantly higher production rates than previous methods.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Separation Science
Background:
- Isoelectric trapping (IET) is a technique for protein separation.
- Preparative-scale enantiomer separation presents challenges in efficiency and speed.
Purpose of the Study:
- To modify the Gradiflow BF200 IET unit for preparative-scale enantiomer separations.
- To evaluate the efficiency and speed of the modified IET unit.
Main Methods:
- Modification of the Gradiflow BF200 IET unit.
- Utilizing hydroxypropyl beta-cyclodextrin as a chiral resolving agent.
- Employing three isoelectric membranes for enantiomer trapping.
Main Results:
- Achieved preparative-scale enantiomer separations.
- Production rates were approximately 15 times higher than a previous IET device.
- Production rates were about 30% higher than a continuous free-flow electrophoretic device.
Conclusions:
- The modified Gradiflow BF200 IET unit demonstrates high efficiency for enantiomer separation.
- The separation speed is attributed to optimized electrophoretic transfer distance, electric field strength, and membrane surface area.