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[Application of reverse transcriptase polymerase chain reaction for surveillance of influenza virus in Beijing]
1Center for Disease Control and Prevention, Beijing 100013, China.
Objective:
To establish the RT-PCR method to type the influenza virus.
Methods:
After amplifying the virus by cell culture, we carried out RT-PCR by using two pairs typing primers and four pairs subtyping primers to detect the influenza virus.
Results:
In those 23 samples which had cytopathologic changes, there were 10 positive strains detected by RT-PCR assay including seven A type (six H3N2 subtype and one H1N1 subtype) and three B type.
Conclusion:
This method is rapid, specific and sensitive and possesses great value for practical application in the surveillance of influenza virus.
