Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lymphocyte surface thiol levels.

Bita Sahaf1, Kartoosh Heydari, Leonard A Herzenberg

  • 1Department of Genetics, Stanford School of Medicine, Stanford, CA 94305-5318, USA. sahaf@darwin.stanford.edu

Proceedings of the National Academy of Sciences of the United States of America
|March 19, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Peripheral Nerve Infiltration by CAR T-cells in a Case of Mononeuritis Multiplex after Ciltacabtagene Autoleucel.

Blood cell therapy·2026
Same author

Post CAR-T Measurable Residual Disease Monitoring in Mantle Cell Lymphoma Enables Early Detection of Disease Relapse.

American journal of hematology·2026
Same author

Randomized, Placebo-Controlled Trial of B-Cell Depletion for Prevention of Corticosteroid-Requiring Chronic Graft-Versus-Host Disease.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2026
Same author

Dynamics of BCMA expression in patients with relapsed/refractory multiple myeloma receiving BCMA-directed CAR-T therapy.

Blood cancer journal·2026
Same author

Early steroid and anakinra use to manage axicabtagene ciloleucel toxicity reduces the total duration of CRS and ICANS.

Blood advances·2026
Same author

Clinical and Cytokine Features of Immune Effector Cell-Associated Hemophagocytic Lymphohistiocytosis-Like Syndrome.

Blood cancer discovery·2025
Same journal

Tau protein as a regulator of mitochondrial function and dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

A scalable, dividing cell model for the robust propagation and quantification of human sporadic Creutzfeldt-Jakob disease prions.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Epigenetic regulation of mesenchymal BMP signaling directs postnatal organ innervation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Single-shot wide-field biochemical imaging at 1 kHz frame rate.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Morphogenesis and topological evolution of a frustrated nematic liquid crystal under confinement.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

B cell-intrinsic CXCR3 drives efficient generation of ectopic pulmonary germinal center responses to influenza A virus infection.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Cell surface thiols vary between immune cells and can be altered by intracellular glutathione (iGSH) in vitro. However, in vivo, the local cellular environment, not iGSH levels, dictates cell surface thiol redox status.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Reduced thiols (cysteine -SH) are implicated in cell surface protein function.
  • Cell surface thiol levels vary across peripheral blood mononuclear cell subsets.
  • Intracellular glutathione (iGSH) can modulate cell surface thiol levels in vitro.

Purpose of the Study:

  • To develop a method for quantifying cell surface thiols.
  • To investigate the relationship between intracellular glutathione and cell surface thiols in vitro and in vivo.
  • To determine factors influencing the redox status of cell surface molecules.

Main Methods:

  • Developed a 11-color fluorescence-activated cell sorter (Hi-D FACS) method using Alexa-maleimide to label free thiols.
  • Quantified cell surface thiols (csm-SH) and intracellular glutathione (iGSH) using fluorescent Alexa dyes.

Related Experiment Videos

  • Analyzed thiol levels in peripheral blood mononuclear cells from healthy and HIV-infected individuals.

    • Main Results:

    • Decreasing iGSH in vitro reduced csm-SH, while preventing iGSH loss preserved csm-SH.
    • No correlation was found between iGSH and csm-SH in freshly isolated cells from healthy or HIV-infected subjects.
    • Significant individual variation in both iGSH and csm-SH was observed.

    Conclusions:

    • Cell surface thiol levels are influenced by the local cellular environment rather than solely by intracellular glutathione levels.
    • The developed Hi-D FACS method provides a novel approach for quantifying cell surface thiols.
    • Further research is needed to elucidate the factors governing cell surface thiol redox status in vivo.