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Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
Published on: April 22, 2022
A DNA strand-nicking principle of a higher plant, Caesalpinia sappan
Woongchon Mar1, Hyun-Tai Lee, Kang-Hoon Je
1Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 110-460, Korea.
Abstract:
To find anticancer agents from higher plants, DNA strand-scission assay method was employed for bioassay-guided fractionation as well as for screening the crude extracts. During the screening, an ethyl acetate extracts of the heartwood of Caesalpinia sappan L. (Leguminosae) exhibited potent DNA strand-scission activity. Therefore, the ethyl acetate extracts of the dried heartwood of C. sappan was subjected to the bioassay-guided fractionation, which led to the isolation of a known compound, brazilin (1) as the active constituent. In addition, caesalpine J (2) was also isolated as an inactive constituent.
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