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Related Experiment Videos

Improved LC method to determine ivermectin in plasma.

J G Prieto1, G Merino, M M Pulido

  • 1Department of Animal Physiology, Veterinary Faculty, University of Leon, Campus of Vegazana s/n, 24007, Leon, Spain. dfijpf@unileon.es

Journal of Pharmaceutical and Biomedical Analysis
|March 20, 2003
PubMed
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A new high-performance liquid chromatographic method accurately quantifies Ivermectin (IVM) in plasma. This rapid assay offers sensitive detection for Ivermectin analysis.

Area of Science:

  • Analytical Chemistry
  • Pharmacology

Background:

  • Accurate quantification of Ivermectin (IVM) in biological matrices is crucial for pharmacokinetic and therapeutic drug monitoring.
  • Existing methods may lack the required sensitivity, speed, or simplicity for routine analysis.

Purpose of the Study:

  • To develop and validate a simple, rapid, and sensitive high-performance liquid chromatographic (HPLC) method for quantifying Ivermectin in plasma.

Main Methods:

  • Developed an isocratic HPLC method with fluorescence detection.
  • Utilized cold methanol for fast liquid-phase extraction.
  • Employed reversed-phase chromatography with a specific mobile phase composition (methanol:acetonitrile:water with 0.2% acetic acid).
  • Fluorescence detection set at 365 nm (excitation) and 475 nm (emission).

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Main Results:

  • Achieved linear calibration for Ivermectin from 0.25 to 100 ng/mL.
  • Demonstrated excellent validation results for linearity, precision, accuracy, specificity, and recoveries.
  • Established a low limit of detection (LOD) of 0.032 ng/mL and limit of quantification (LOQ) of 0.167 ng/mL.

Conclusions:

  • The developed HPLC method is suitable for the sensitive and accurate quantification of Ivermectin in plasma.
  • The method's simplicity and speed make it advantageous for routine laboratory analysis.
  • This assay provides a valuable tool for Ivermectin research and clinical applications.