Conductometric and indirect AAS determination of antimalarials

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Accurate quantification of antimalarial drugs is crucial for quality control.
• Existing analytical methods may require improvement in sensitivity or applicability.

Purpose of the Study:

• To develop and validate novel analytical methods for amodiaquine hydrochloride, chloroquine phosphate, and primaquine phosphate.
• To establish ion-association-based conductometric and atomic absorption spectrometric procedures.

Main Methods:

• Formation of ion-associates between antimalarial drugs and metal thiocyanate, ammonium reineckate, or sodium cobaltinitrite reagents.
• Direct determination using conductometry and indirect determination using atomic absorption spectrometry (AAS).
• Optimization of ion-association conditions and determination of molar ratios.

Main Results:

• Established (1:1) and (1:2) drug:reagent molar ratios for ion-associate formation.
• Defined optimal conditions for conductometric and indirect AAS methods.
• Achieved optimal concentration ranges of 0.46-12.90 mg (conductometric) and 0.155-3.87 mg (indirect AAS).
• Successfully applied methods to pharmaceutical formulations with comparable results to official methods.

Conclusions:

• The proposed conductometric and indirect AAS methods are effective for the quantitative analysis of amodiaquine hydrochloride, chloroquine phosphate, and primaquine phosphate.
• These methods provide reliable and accurate results for both bulk drugs and pharmaceutical formulations.
• The ion-association approach offers a viable alternative for antimalarial drug determination.