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Updated: Sep 26, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
Multi locus fingerprinting of Listeria monocytogenes by sequence-specific labeling of DNA probes combined with array
Knut Rudi1, Tone Katla, Kristine Naterstad
1MATFORSK, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway. knut.rudi@matforsk.no
Abstract:
We have developed an alternative multi locus sequence typing (MLST) approach that targets the variable genetic changes directly in a DNA array format. Our approach is based on DNA array hybridization in combination with sequence-specific labeling of oligonucleotide probes. Listeria monocytogenes was chosen for the development and evaluation of the assay. The genes hlyA, iap, flaA, inlA and actA were targeted. Twenty-nine suitable probe regions were identified within these genes. The DNA array results from 32 different strains were compared to serotype and amplified fragment length polymorphism data. This comparison showed that our DNA array method gave good discrimination between the strains analyzed. In conclusion, the DNA array-based MLST method is a promising tool for fingerprint bacteria.
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