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Related Experiment Videos

Gene expression of EXT1 and EXT2 during mouse brain development.

Masaru Inatani1, Yu Yamaguchi

  • 1The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.

Brain Research. Developmental Brain Research
|March 20, 2003
PubMed
Summary

Heparan sulfate (HS) synthesis enzymes EXT1 and EXT2 are crucial for brain development. Their expression patterns in mice brains reveal significant roles in neurodevelopmental processes.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Biochemistry

Background:

  • Heparan sulfate (HS) and heparan sulfate proteoglycans (HSPGs) are vital in biological processes.
  • Evidence suggests HS involvement in mammalian neural development.

Purpose of the Study:

  • To investigate the spatiotemporal expression of EXT1 and EXT2 during mouse brain development.
  • To understand the role of HS in neural development through its synthesis enzymes.

Main Methods:

  • Reverse transcription polymerase chain reaction (RT-PCR) to analyze gene expression.
  • In situ hybridization to determine the localization of EXT1 and EXT2 expression.

Main Results:

  • EXT1 and EXT2 expression peaked in the early postnatal cerebrum and around birth in the cerebellum.

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  • Expression was observed in neuroepithelial cells, cerebral cortex, hippocampus, and cerebellar granular layers.
  • Expression patterns were developmentally regulated.

    • Conclusions:

    • EXT1 and EXT2 are highly expressed during mouse brain development.
    • The developmental regulation of EXT1 and EXT2 suggests HS involvement in neurodevelopmental processes.