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Related Experiment Video

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Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach

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Cloning, expression, and characterization of chicken transforming growth factor beta 4.

Hongjie Pan¹, Jaroslava Halper

¹The Soft Tissue Center, Department of Pathology, College of Veterinary Medicine, The University of Georgia, GA, Athens 30602-7388, USA.

Biochemical and Biophysical Research Communications

|March 21, 2003

Summary

This summary is machine-generated.

Transforming growth factor beta 4 (TGF-beta 4), unique to birds, was expressed and characterized. This avian TGF-beta 4 inhibits cell growth and stimulates collagen and heat shock protein production, suggesting similar roles to mammalian TGF-beta 1.

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Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

Transforming growth factor beta 4 (TGF-beta 4) is unique to avian species.
Its in vivo roles remain largely unestablished.

Purpose of the Study:

To express and partially characterize recombinant chicken TGF-beta 4.
To investigate the biological activities of avian TGF-beta 4.

Main Methods:

Generated 5'-end cDNA sequence using a modified 5'RACE methodology.
Cloned and transfected into Chinese hamster ovary (CHO-K1) cells for stable expression.
Tested biological activity on mink lung epithelial (Mv1Lu) and chicken tendon fibroblasts.

Main Results:

Established a CHO-K1 cell line stably expressing TGF-beta 4 precursor.
Acid-activated mature TGF-beta 4 inhibited Mv1Lu cell growth.
TGF-beta 4 stimulated type I procollagen and heat shock protein 47 (Hsp47) expression in chicken tendon fibroblasts, likely via HSF1 activation.

Conclusions:

Avian TGF-beta 4 elicits biological activities in chicken tendon cells similar to mammalian TGF-beta 1.
TGF-beta 4 likely plays analogous roles to TGF-beta 1 in avian species.
This study provides insights into the function of TGF-beta 4 in birds.