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The appearance of truncated cyclin A2 correlates with differentiation of mouse embryonic stem cells

Martin Anger1, Vitezslav Bryja, Ludmila Jirmanova

  • 1Department of Biology, University of Pennsylvania, USA.

Biochemical and Biophysical Research Communications
|March 21, 2003
PubMed

Insights

Truncated cyclin A2 increases during mouse embryonic stem cell differentiation, independent of p27(Kip1). This post-translational modification correlates with differentiation, not cell density.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • A truncated form of cyclin A2, with an N-terminal truncation, has been identified in murine cells.
  • This truncated cyclin A2 interacts with and activates cyclin-dependent kinase 2 (CDK2).
  • Unlike full-length cyclin A2-bound CDK2 (nuclear), truncated cyclin A2-bound CDK2 localizes to the cytoplasm.

Purpose of the Study:

  • To investigate the expression pattern of truncated cyclin A2 during mouse embryonic stem cell differentiation.
  • To determine the role of p27(Kip1) in the generation of truncated cyclin A2.
  • To ascertain whether cell density influences the production of truncated cyclin A2.

Main Methods:

  • Analysis of truncated cyclin A2 levels in proliferating and differentiating mouse embryonic stem (ES) cells.
  • Comparison of truncated cyclin A2 expression in wild-type and p27(Kip1) knockout (p27(Kip1) -/-) differentiating ES cells.
  • Correlation analysis between truncated cyclin A2 levels, cell differentiation, and cell density.

Main Results:

  • Proliferating mouse ES cells exhibit minimal truncated cyclin A2.
  • Truncated cyclin A2 levels significantly increase upon induction of ES cell differentiation.
  • The expression pattern of truncated cyclin A2 during differentiation is unaffected by the absence of p27(Kip1).

Conclusions:

  • The generation of truncated cyclin A2 in differentiating ES cells is not dependent on p27(Kip1).
  • The post-translational modification leading to truncated cyclin A2 is linked to the differentiation process.
  • Cell density does not appear to be a determining factor for truncated cyclin A2 production; differentiation status is the key correlate.

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