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Related Experiment Videos

B cell complement receptor 2 transfer reaction.

Margaret A Lindorfer1, Hasmig B Jinivizian, Patricia L Foley

  • 1Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908-0733, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|March 21, 2003
PubMed
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Immune complexes (IC) bound to B cell complement receptor 2 (CR2) can be transferred to other cells, along with CR2 itself. This transfer process, observed in vitro and in vivo, sheds light on how the complement system handles ICs.

Area of Science:

  • Immunology
  • Complement System Biology
  • Cellular Immunology

Background:

  • The B cell complement receptor 2 (CR2) interacts with C3dg, a fragment of the complement system's C3 protein.
  • Previous research demonstrated immune complex (IC) transfer from primate erythrocytes (E) via complement receptor 1 (CR1) to macrophages.
  • This erythrocyte-based transfer process involves the removal of CR1 from the E.

Purpose of the Study:

  • To investigate whether a similar IC transfer reaction occurs with B cell CR2.
  • To characterize the mechanism and cellular requirements of IC transfer involving CR2.
  • To explore the in vivo relevance of CR2-mediated IC handling.

Main Methods:

  • Utilized flow cytometry and fluorescence microscopy to analyze IC transfer.

Related Experiment Videos

  • Employed Raji cells expressing CR2 and THP-1 cells as acceptor cells.
  • Investigated the role of complement opsonization and anti-CR2 monoclonal antibodies (mAbs) in binding ICs to CR2.
  • Conducted a prototype study in a monkey model.

    • Main Results:

    • Demonstrated that ICs bound to Raji cell CR2 are transferred to acceptor THP-1 cells.
    • Confirmed transfer of CR2 along with the ICs.
    • Showed that the transfer reaction requires Fc recognition of IgG bound to CR2.
    • Observed localization of ICs bound to B cell CR2 in the spleen in a monkey model.

    Conclusions:

    • The transfer reaction paradigm extends to B cell CR2, involving IC and CR2 transfer to acceptor cells.
    • Fc-mediated recognition is crucial for this B cell transfer reaction.
    • Findings suggest a potential role for CR2-mediated IC handling in normal immune responses and complement function.