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Monocyte function in preterm, term, and adult sheep.

Boris W Kramer1, Alan H Jobe, Machiko Ikegami

  • 1Cincinnati Children's Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, OH 45229-3039, USA.

Pediatric Research
|March 21, 2003
PubMed
Summary

Monocytes in preterm infants are immature, showing reduced inflammatory responses and impaired phagocytosis. Interventions like dexamethasone are less effective, potentially prolonging inflammation in conditions such as bronchopulmonary dysplasia.

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Area of Science:

  • Immunology
  • Neonatal Medicine
  • Developmental Biology

Background:

  • Preterm infants possess functionally immature monocytes, impacting their immune responses.
  • Understanding monocyte function is crucial for managing neonatal inflammatory conditions.

Purpose of the Study:

  • To evaluate the effects of clinical interventions and exposures on monocyte function in preterm lambs compared to adult sheep.
  • To investigate the differences in inflammatory initiation and resolution between preterm and adult monocytes.

Main Methods:

  • Monocytes were isolated from preterm lambs (130 d gestational age), near-term lambs (141 d GA), and adult sheep.
  • Monocyte responses to endotoxin, dexamethasone, and surfactants were assessed, measuring hydrogen peroxide production, apoptosis, and phagocytosis.

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Main Results:

  • Monocytes from preterm and near-term lambs exhibited reduced and delayed hydrogen peroxide production in response to endotoxin compared to adults.
  • Endotoxin did not decrease apoptosis in preterm/near-term lamb monocytes, unlike adult monocytes.
  • Dexamethasone enhanced phagocytosis in adult monocytes but not in preterm/near-term lamb monocytes.
  • Surfactants increased apoptotic cell phagocytosis across all groups.

Conclusions:

  • Monocytes from preterm and term lambs display distinct functional deficits in both initiating and resolving inflammation compared to adult monocytes.
  • Impaired phagocytosis of apoptotic cells by preterm monocytes may contribute to sustained inflammation in diseases like bronchopulmonary dysplasia.