Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The road towards faster development of novel pharmacotherapies for persons with Parkinson's disease.

Journal of Parkinson's disease·2026

Same author

Modulating α-synuclein clearance: a comparison of high-frequency and direct current stimulation in preclinical models of Parkinson's disease.

Brain communications·2026

Same author

Phase 2 Futility Trials in Alzheimer Disease and Mild Cognitive Impairment: A Cohort Analysis of the ADNI Data Set.

Neurology·2026

Same author

Deep brain stimulation in alpha-synuclein models of Parkinson's disease: Bridging the translational gap.

Journal of Parkinson's disease·2026

Same author

Georgina M. Aldridge, Matthew Weber and Lauren Walker recipients of the Parkinson Prize 2025.

Journal of Parkinson's disease·2026

Same author

Co- and Multi-Pathologies in Parkinson's Disease: An International Parkinson and Movement Disorder Society Scientific Issues Committee Review.

Movement disorders : official journal of the Movement Disorder Society·2026

Search research articles

Related Experiment Video

Updated: Apr 9, 2026

Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors

Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors

Published on: November 14, 2014

Glycine binding primes NMDA receptor internalization.

Yi Nong¹, Yue-Qiao Huang, William Ju

¹Programme in Brain and Behaviour, Hospital for Sick Children, , University of Toronto, Toronto, Ontario M5G 1X8, Canada.

|March 21, 2003

Summary

Glycine binding to NMDA receptors (NMDARs) primes them for internalization, but requires glutamate for full endocytosis. This reveals a new signaling pathway for NMDARs in the central nervous system.

More Related Videos

$Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala$

Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala

Published on: April 15, 2016

An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

Published on: August 2, 2019

Related Experiment Videos

Last Updated: Apr 9, 2026

Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors

Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors

Published on: November 14, 2014

$Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala$

Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala

Published on: April 15, 2016

An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

Published on: August 2, 2019

Area of Science:

Neuroscience
Molecular Biology
Cell Signaling

Background:

NMDA receptors (NMDARs) are crucial excitatory ion channels in the central nervous system.
Glycine acts as a co-agonist, essential for NMDAR activation alongside glutamate.
Understanding NMDAR regulation is key to comprehending synaptic communication and neurological disorders.

Purpose of the Study:

To investigate the role of glycine binding in NMDAR signaling and trafficking.
To elucidate the mechanism by which glycine site activation influences NMDAR endocytosis.
To explore the implications of glycine-mediated signaling for neuronal communication.

Main Methods:

Utilized NMDAR glycine site agonists and antagonists.
Investigated receptor priming for clathrin-dependent endocytosis.
Examined both synaptic and extrasynaptic NMDARs.

Main Results:

Glycine site stimulation primes NMDARs for endocytosis, a process requiring subsequent glutamate binding.
D-serine, an NMDAR glycine site agonist, mimics this priming effect.
Glycine site antagonists block this priming mechanism, affecting synaptic and extrasynaptic NMDARs.

Conclusions:

Glycine binding initiates transmembrane signal transduction through the NMDAR complex.
This priming mechanism provides a novel model for modulating NMDAR function and cell-cell communication.
Findings highlight the distinct roles of glycine and glutamate in regulating NMDAR trafficking and signaling.