Related Experiment Videos
[Gene expression profiling in prostatic cancer]
T Ernst1, M Hergenhahn, M Kenzelmann
1Abteilung Zelluläre und Molekulare Pathologie, Deutsches Krebsforschungszentrum (DKFZ) Heidelberg.
Verhandlungen Der Deutschen Gesellschaft Fur Pathologie
|March 22, 2003
Summary
Researchers analyzed gene expression in prostate cancer tissues, identifying over 200 genes with altered expression. These findings may lead to new diagnostic and prognostic markers for prostate cancer.
Area of Science:
- Molecular Biology
- Oncology
- Genomics
Context:
- Prostate carcinoma biology and molecular alterations are not fully understood.
- Current histopathological diagnosis may benefit from additional diagnostic and prognostic markers.
- Gene expression profiling offers a method to identify novel markers.
Purpose:
- To perform a comprehensive gene expression analysis on non-metastasizing, untreated prostate cancer tissues.
- To identify differentially expressed genes in prostate adenocarcinoma compared to normal adjacent tissues.
- To distinguish gene expression profiles between epithelial and stromal compartments.
Summary:
- Utilized high-density oligonucleotide microarrays to analyze RNA expression profiles of approximately 12,600 sequences from 26 human prostate tissues.
- Identified 63 significantly upregulated and 153 downregulated genes, including novel and previously reported genes.
- Laser capture microdissection confirmed that most upregulated genes were overexpressed in cancerous epithelium.
Impact:
- Demonstrated that prostate cancer development involves complex differential gene regulation in both epithelial and stromal compartments.
- Identified potential novel diagnostic and therapeutic targets for prostate cancer.
- Gene expression profiling of microdissected tumor cells may supplement histopathological diagnosis.