Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural information about organized cholesterol domains from specific antibody recognition.

Lia Addadi1, Merav Geva, Howard S Kruth

  • 1Department of Structural Biology, Weizmann Institute of Science, 76100 Rehovot, Israel. Lia.Addadi@weizmann.ac.il

Biochimica Et Biophysica Acta
|March 22, 2003
PubMed
Summary

Cholesterol-rich membrane domains are crucial but poorly understood. This study explores using specialized antibodies to reveal their molecular organization within cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biomineralization: Perspectives on control of crystal polymorphism, order-disorder and solvation states.

Quarterly reviews of biophysics·2026
Same author

Solving the Crystal Architecture of Coccoliths Using 4D-STEM.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2025
Same author

3D cryoimaging of cell-mediated cholesterol crystal clearance in human atherosclerotic lesions.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Guanine crystal formation by the unicellular organism Phacotus lenticularis is part of a cellular stress response.

PloS one·2025
Same author

Organic Crystals and Optical Functions in Biology: Knowns and Unknowns.

Advanced materials (Deerfield Beach, Fla.)·2024
Same author

Bio-Inspired Crystalline Core-Shell Guanine Spherulites.

Advanced materials (Deerfield Beach, Fla.)·2024

Area of Science:

  • Cell Biology
  • Membrane Biophysics
  • Immunology

Background:

  • Cholesterol-rich domains are present in cell membranes under various conditions.
  • Their formation and composition vary widely.
  • Limited information exists on the molecular structure and organization of these domains.

Purpose of the Study:

  • To review techniques for analyzing membrane domain structure.
  • To explore the potential of tailor-made antibodies as reporters of molecular organization.
  • To establish the concept of antibodies recognizing molecular organization, not just single epitopes.

Main Methods:

  • Review of existing techniques for structural analysis of membrane domains.
  • Conceptualization and review of antibody-protein recognition of crystalline molecular arrays.

Related Experiment Videos

  • Examination of antibody-based reporters for molecular organization in cellular membranes.

    • Main Results:

    • Existing techniques for membrane domain structural analysis are limited.
    • Antibodies can be engineered to recognize molecular organization within membranes.
    • This approach offers a novel method for studying domain structure.

    Conclusions:

    • Tailor-made antibodies represent a promising tool for investigating molecular organization in cholesterol-rich membrane domains.
    • This method provides a new avenue for understanding the structure and function of these critical membrane components.