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In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System
Published on: November 24, 2015
CD8alpha+ mouse spleen dendritic cells do not originate from the CD8alpha- dendritic cell subset
Shalin Naik1, David Vremec, Li Wu
1The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Blood
|March 22, 2003
Summary
Murine dendritic cells (DCs) subtypes CD8alpha- and CD8alpha+ are not directly related as precursor and product. Most CD8alpha+ DCs originate from unrecognized precursor cells, challenging previous findings.
Area of Science:
- Immunology
- Cell Biology
Background:
- Dendritic cells (DCs) are crucial immune cells.
- Murine CD8alpha- and CD8alpha+ DCs are distinct subtypes.
- Previous research suggested a direct precursor-product relationship between CD8alpha- and CD8alpha+ DCs.
Purpose of the Study:
- To investigate the precursor-product relationship between CD8alpha- and CD8alpha+ dendritic cells (DCs) in mice.
- To clarify the origin of CD8alpha+ DCs.
Main Methods:
- Intravenous transfer of CD8alpha- DCs into recipient mice.
- Balance sheet approach to track DC populations.
- Phenotypic analysis of dendritic cell precursors.
Main Results:
- Intravenous transfer of CD8alpha- DCs rarely generated CD8alpha+ DCs.
- The majority of CD8alpha+ DCs originated from precursor cells lacking a recognizable DC phenotype.
- CD8alpha- and CD8alpha+ DCs are not directly precursor-product related.
Conclusions:
- The direct precursor-product relationship between CD8alpha- and CD8alpha+ DCs is unlikely.
- CD8alpha+ DCs arise from uncharacterized precursor cells.
- These DC sublineages may be connected further upstream in their development.
