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Updated: Jul 9, 2026
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Generation of Genetically Modified Mice through the Microinjection of Oocytes
Published on: June 15, 2017
Strain-dependent differences in the efficiency of transgenic mouse production
Anna B Auerbach1, Rada Norinsky, Weilan Ho
1Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, NYU School of Medicine, 540 First Avenue, New York, NY 10016, USA. anauerba@saturn.med.nyu.edu
Transgenic Research
|March 26, 2003
Summary
Choosing the right mouse strain significantly impacts transgenic mouse production efficiency. FVB/N mice yield the highest success rates across all production steps, offering a superior model for generating genetically modified organisms.
Area of Science:
- * Developmental Biology
- * Genetics
- * Animal Models
Background:
- * Transgenic mouse production is crucial for biological research, but its efficiency is affected by numerous factors.
- * Key steps include superovulation response, fertilization, egg survival post-injection, embryo implantation, and DNA quality.
- * Selecting appropriate egg donor strains is critical for optimizing the overall process.
Purpose of the Study:
- * To evaluate and compare the efficiency of transgenic mouse production across four distinct egg donor mouse strains.
- * To identify strain-specific limitations and advantages in the transgenic workflow.
- * To provide data-driven recommendations for enhancing transgenic mouse generation.
Main Methods:
- * Comparative analysis of transgenic production efficiency using B6D2/F1 hybrids, Swiss Webster (SW), FVB/N, and C57BL/6 egg donor strains.
- * Data compiled from approximately 350 DNA transgene construct experiments.
- * Evaluation of efficiency at each sequential step of pronuclear microinjection and subsequent development.
Main Results:
- * FVB/N mice demonstrated the highest efficiency in transgenic mouse production across all steps, excluding egg production.
- * B6D2/F1 hybrid eggs showed high efficiency but increased lysis post-microinjection.
- * SW eggs exhibited higher rates of 1-cell stage arrest, while C57BL/6 had lower fetal development to term.
Conclusions:
- * Genetic background significantly influences the efficiency of each stage in transgenic mouse production.
- * FVB/N mice are the most efficient strain for overall transgenic production.
- * Tailoring microinjection procedures to specific donor strains can overcome deficiencies and improve success rates.
