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Strain-dependent differences in the efficiency of transgenic mouse production.

Anna B Auerbach1, Rada Norinsky, Weilan Ho

  • 1Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, NYU School of Medicine, 540 First Avenue, New York, NY 10016, USA. anauerba@saturn.med.nyu.edu

Transgenic Research
|March 26, 2003
PubMed
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Choosing the right mouse strain significantly impacts transgenic mouse production efficiency. FVB/N mice yield the highest success rates across all production steps, offering a superior model for generating genetically modified organisms.

Area of Science:

  • * Developmental Biology
  • * Genetics
  • * Animal Models

Background:

  • * Transgenic mouse production is crucial for biological research, but its efficiency is affected by numerous factors.
  • * Key steps include superovulation response, fertilization, egg survival post-injection, embryo implantation, and DNA quality.
  • * Selecting appropriate egg donor strains is critical for optimizing the overall process.

Purpose of the Study:

  • * To evaluate and compare the efficiency of transgenic mouse production across four distinct egg donor mouse strains.
  • * To identify strain-specific limitations and advantages in the transgenic workflow.
  • * To provide data-driven recommendations for enhancing transgenic mouse generation.

Main Methods:

  • * Comparative analysis of transgenic production efficiency using B6D2/F1 hybrids, Swiss Webster (SW), FVB/N, and C57BL/6 egg donor strains.

Related Experiment Videos

  • * Data compiled from approximately 350 DNA transgene construct experiments.
  • * Evaluation of efficiency at each sequential step of pronuclear microinjection and subsequent development.

    • Main Results:

    • * FVB/N mice demonstrated the highest efficiency in transgenic mouse production across all steps, excluding egg production.
    • * B6D2/F1 hybrid eggs showed high efficiency but increased lysis post-microinjection.
    • * SW eggs exhibited higher rates of 1-cell stage arrest, while C57BL/6 had lower fetal development to term.

    Conclusions:

    • * Genetic background significantly influences the efficiency of each stage in transgenic mouse production.
    • * FVB/N mice are the most efficient strain for overall transgenic production.
    • * Tailoring microinjection procedures to specific donor strains can overcome deficiencies and improve success rates.