Practical considerations in refolding proteins from inclusion bodies
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Updated: Sep 26, 2026

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Published on: December 12, 2017
Kouhei Tsumoto1, Daisuke Ejima, Izumi Kumagai
1Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Japan.
Abstract:
Refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. We will review key parameters associated with (1) conformation of the protein solubilized from inclusion bodies, (2) change in conformation and flexibility or solubility of proteins during refolding upon reduction of denaturant concentration, and (3) the effect of small molecule additives on refolding and aggregation of the proteins.
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