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Molecular cloning and characterization of mannan binding lectin C gene from Balb/c mice
Hong-wei Wang1, Zheng-liang Chen, Da-ming Zuo
1Department of Immunology, First Military Medical University, Guangzhou 510515, China. whwnet@fimmu.com
Objective:
To obtain full-length cDNA encoding mouse mannan-binding lectin C(MBL-C) polypeptide.
Methods:
The cDNA encoding mouse MBL-C was isolated from Balb/c mouse liver cells by reverse transcriptase (RT)-PCR and inserted into pUC-T vector, and the encoding region structure of the DNA was analyzed to understand its evolutionary relationship with single human MBL homologues.
Results:
The amplified mouse MBL-C cDNA, which was 735 bp in length, encoded 245 amino acid residues, and its structural analysis showed 100% homology with published mouse MBL-C gene sequence and 71.4% homology with MBL gene of Chinese human.
Conclusion:
The gene encoding Balb/c mouse MBL-C has been successfully isolated, which may facilitate further study of the innate immune functions of MBL molecule in vivo.