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Updated: Sep 26, 2026

Ganglioside Extraction, Purification and Profiling
Published on: March 12, 2021
Structure of the main ganglioside from the brain of Xenopus laevis
Angela Maria Rizzo1, Bruno Berra, Federica Rossi
1Institute of General Physiology and Biochemistry, 20134 Milan, Italy.
Abstract:
The main component of the ganglioside(1) mixture from the brain of the adult amphibian Xenopus laevis accounts for 35% of the total, as lipid bound sialic acid. This ganglioside has been purified and characterized by thin layer chromatography, partial and exhaustive enzymatic hydrolysis with sialidase, TLC-overlay procedures with anti-Gg(4)Cer and anti-Neu5Acalpha6GalNAcbeta specific monoclonal antibodies and mass spectrometry. All together the results suggest the following structure: Neu5Acalpha8Neu5Acalpha3Galbeta3(Neu5Acalpha8Neu5Acalpha6)GalNAcbeta4Galbeta4Glcbeta1Cer, or, IV(3)-alpha-Neu5Ac(2),III(6)-alpha-Neu5Ac(2)-Gg(4)Cer.
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