Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Accelerated process development for protease production in continuous multi-stage cultures.

A Raninger1, W Steiner

  • 1Department of Biochemical Reaction Engineering, Institute of Biotechnology-SFB Biocatalysis, University of Technology Graz, Petersgasse 12, 8010 Graz, Austria. alexander.raninger@gmx.net

Biotechnology and Bioengineering
|March 26, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wildlife-vehicle collision liability in Europe: A review of existing approaches and their implications.

Journal of environmental management·2025
Same author

Mössbauer investigations of crystalline and quasicrystalline Al3(Mn, Fe) compounds.

Journal of physics. Condensed matter : an Institute of Physics journal·2011
Same author

Electronic structure of a σ-FeCr compound.

Journal of physics. Condensed matter : an Institute of Physics journal·2011
Same author

A Case of Rat Bite Fever.

Transactions of the American Climatological and Clinical Association. American Climatological and Clinical Association·2011
Same author

[Incidence, latency, and prognosis of local and regional recurrences in patients with upper aerodigestive tract cancer treated by laser microsurgery: influence of initial tumor parameters].

HNO·2007
Same author

Incompatibility and pollen competition in Alnus glutinosa: Evidence from pollination experiments.

Genetica·2005

Researchers optimized a fermentation process for Bacillus licheniformis PWD-1 protease production. This enhanced fed-batch and continuous culture methods, achieving a tenfold increase in protease activity for potential pilot-scale enzyme production.

Area of Science:

  • Biotechnology and Industrial Microbiology
  • Enzyme Production
  • Fermentation Process Optimization

Background:

  • Bacillus licheniformis PWD-1 is a known producer of valuable proteases.
  • Optimizing fermentation is crucial for efficient and cost-effective enzyme production.
  • Existing batch processes may limit protease yield and production scalability.

Purpose of the Study:

  • To develop and optimize a fermentation process for enhanced protease production from Bacillus licheniformis PWD-1.
  • To investigate and control key environmental parameters influencing microbial growth and enzyme formation.
  • To establish a scalable continuous production method for the target protease.

Main Methods:

  • Optimization of media formulations and environmental parameters (e.g., temperature, pH).

Related Experiment Videos

  • Kinetic analysis of substrate consumption, biomass, product, and by-product formation in batch and continuous cultures.
  • Development of fed-batch and two-stage continuous stirred-tank reactor (CSTR) fermentation strategies.

    • Main Results:

    • A fed-batch process yielded protease activities 10 times higher than standard batch cultures.
    • Protease formation was successfully decoupled from sporulation in a single-stage continuous culture.
    • A two-stage continuous fermentation process was developed, optimizing growth and protease production separately.

    Conclusions:

    • The optimized fermentation strategies significantly enhance protease yield from Bacillus licheniformis PWD-1.
    • Decoupling protease formation from sporulation and utilizing continuous culture improves production efficiency.
    • The developed two-stage continuous process provides a foundation for pilot-scale enzyme manufacturing.