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Updated: Sep 26, 2026

Lensless Fluorescent Microscopy on a Chip
Published on: August 17, 2011
An interface chip connection between capillary electrophoresis and thermal lens microscope
Kenji Uchiyama1, Akihide Hibara, Kiichi Sato
1Integrated Chemistry Project, Kanagawa Academy of Science and Technology, Kawasaki, Japan.
Abstract:
A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused-silica capillaries with an inner diameter of 50 microm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8 x 10(-7) absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE-TLM was estimated as 2.4 x 10(-8) M, which was 100 times lower than that of conventional absorbance detection.
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