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Updated: Aug 19, 2026

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
Published on: April 3, 2018
Cyclic guanosine monophosphate phosphodiesterase activity in human gingival carcinoma
Giuseppe Spoto1, Massimiliano Fioroni, Corrado Rubini
1Department of Applied Sciences of Oral and Dental Diseases, G. D'Annunzio University, Via dei Vestini 31, 66013 Chieti, Italy. spoto@unich.it
Background:
Cyclic guanosine monophosphate (cGMP) is an essential second messenger metabolized by phosphodiesterases (PDEs).
Objectives:
We looked for a possible correlation of PDE activities in human oral squamous cell carcinoma (OSCC) with and without lymph node metastases.
Materials And Methods:
The analysis of phosphodiesterase activity and the cGMP assay were done by reverse-phase HPLC on samples of fresh or frozen gingival tissues. Analysis of cGMP was confirmed with the enzyme-linked immunoabsorption assay.
Results And Conclusions:
cGMP PDE activity was 34.92 +/- 7.17 SD, 12.89 +/- 4.43 SD, and 35.88 +/- 8.76 SD (nmols/mg of protein), respectively, in controls, samples without lymph node involvement (N-), and specimens with lymph node metastases (N+). cGMP values were 1.97 +/- 0.63 SD, 3.30 +/- 1.47 SD, and 3.49 +/- 1.47 SD (nmols/mg of protein). Our data support the hypothesis of a role for cGMP and PDE in the progression of OSCC.
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