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Updated: Aug 11, 2026
10:44
One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
Published on: June 18, 2016
Helicobacter pylori and Campylobacter rectus share a common antigen
S Tanabe1, D Hinode, M Yokoyama
1Department of Preventive Dentistry, School of Dentistry, The University of Tokushima, Tokushima City, Japan.
Oral Microbiology and Immunology
|March 26, 2003
Summary
This study found shared antigens between Helicobacter pylori and Campylobacter rectus, specifically a heat shock protein 60 (HSP60) called GroEL. This shared protein may contribute to periodontal disease development.
Area of Science:
- Microbiology
- Immunology
- Oral Health
Background:
- Periodontopathogenic bacteria and Helicobacter pylori cause distinct diseases.
- Investigating shared antigens could reveal cross-reactivity and potential disease links.
Purpose of the Study:
- To identify immunological cross-reactivity between periodontopathogens and H. pylori.
- To characterize shared antigens and their potential role in disease.
Main Methods:
- SDS-PAGE/Western immunoblotting using antibodies against H. pylori.
- N-terminal amino acid sequencing and nucleotide sequencing of identified antigens.
- Assessing the biological activity of the identified antigen on human gingival epithelial cells.
Main Results:
- Cross-reactive antigens were detected in Campylobacter rectus using H. pylori antibodies.
- A 64-kDa antigen in C. rectus was identified as a GroEL protein (HSP60 family).
- C. rectus GroEL showed 76.8% amino acid similarity to H. pylori GroEL and stimulated IL-6 production.
Conclusions:
- An immunological relationship exists between H. pylori and C. rectus.
- Shared GroEL protein in C. rectus may contribute to periodontal disease initiation and progression.
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