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[Tumor necrosis factor alpha HLA-DRB(1) gene polymorphism and genetic susceptibility to cirrhosis]
Jusheng Lin1, Yuanqiao Cheng, Deying Tian
1The Institute of Liver Disease, Tongji Hospital, Tongji Medical College, University of Huazhong Science and Technology, Wuhan 430030, China. linjusheng2001@163.net
Insights
Genetic variations in HLA-DRB1 and tumor necrosis factor alpha influence cirrhosis susceptibility. Specifically, HLA-DRB1*120X and TNF2 alleles increase risk, while HLA-DRB1*150X may offer protection against liver cirrhosis.
Area of Science:
- Immunogenetics
- Hepatology
- Molecular Biology
Context:
- Cirrhosis, a severe liver condition, has complex etiologies.
- Genetic factors play a role in an individual's susceptibility to developing cirrhosis.
- Hepatitis B virus (HBV) is a significant cause of cirrhosis globally.
Purpose:
- To investigate the association between specific gene polymorphisms of HLA-DRB1 and tumor necrosis factor (TNF) alpha and the genetic susceptibility to cirrhosis.
- To identify potential genetic markers that confer risk or protection against HBV-induced liver cirrhosis.
Summary:
- This study analyzed the gene polymorphisms of HLA-DRB1 and TNF alpha in 106 HBV-related cirrhosis patients and 108 controls using PCR-SSP and RFLP.
- Increased frequencies of HLA-DRB1*120X and TNF2 alleles were observed in cirrhosis patients compared to controls.
- Conversely, the HLA-DRB1*150X allele was less frequent in patients, suggesting a protective role.
Impact:
- The findings suggest that HLA-DRB1*120X and TNF2 alleles are associated with an increased risk of developing liver cirrhosis.
- HLA-DRB1*120X may act as a susceptibility gene, while HLA-DRB1*150X may function as a protective gene.
- These genetic insights contribute to understanding the pathogenesis of cirrhosis and may inform future risk assessment strategies.
Objective:
To study the relationship between the gene polymorphism of HLA-DRB(1) and tumor necrosis factor (TNF)alpha with the genetic susceptibility to cirrhosis.
Methods:
The gene polymorphism of DRB(1) and TNF alpha of 106 cases of cirrhosis due to HBV and 108 controls were detected by means of polymerase chain reaction-sequence specific primer and RFLP techniques.
Results:
The frequency of DRB(1) * 120X and TNF2/1 was increased in the patients as compared with the controls (35.9% vs 11.1%, P < 0.001, 19.8% vs 10.2%, P < 0.05 respectively), The frequency of DRB(1) * 150X allele was reduced in the patients as compared with the controls (13.2% vs 30.6%, P < 0.05). It is suggested that the gene polymorphism of HLA-DRB(1) and TNF alpha to be associated with genetic susceptibility to cirrhosis. Cross analysis showed that DRB(1) * 120X allele was more strongly associated with genetic susceptibility to cirrhosis than TNF2 allele.
Conclusions:
The genetic susceptibility to cirrhosis is associated with DRB(1) * 120X and TNF2 allele, persons with DRB(1) * 120X and TNF2 allele have an increased risk for the liver cirrhosis occurrence; DRB(1) * 120X allele may be a susceptibility gene to liver cirrhosis and DRB(1) * 150X allele may be a protective gene from liver cirrhosis.