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Rheumatoid factor appearance in Micrococcus lysodeikticus immunization and its interference with allotype specific
Zeitschrift Fur Immunitatsforschung, Experimentelle Und Klinische Immunologie
|July 1, 1975
Summary
Rabbit rheumatoid factors can interfere with immune reactions, causing false positives in immunodiffusion tests. This study shows how to use this interference to accurately type rabbit allotypes d12 and A8.
Area of Science:
- Immunology
- Rabbit genetics
Background:
- Hyperimmunization of rabbits with Micrococcus lysodeikticus induces anti-cell wall antibodies.
- These rabbits also produce anti-immunoglobulins, known as rheumatoid factors, which can complicate immune assays.
Purpose of the Study:
- To investigate the interference of rheumatoid factors in immunodiffusion assays.
- To develop a method for typing rabbit allotypes d12 and A8 in the presence of rheumatoid factors.
Main Methods:
- Hyperimmunization of rabbits with Micrococcus lysodeikticus.
- Immunodiffusion assays to detect antibody reactions.
- Utilizing rheumatoid factor-induced visible reactions for allotype typing.
Main Results:
- Rabbit rheumatoid factors can cause non-precipitating systems in immunodiffusion.
- Specific rabbit allotypes (d12 and A8) show visible reactions in the presence of rheumatoid factors.
- Inhibition of these reactions allows for typing even in sera lacking rheumatoid factors.
Conclusions:
- Rabbit rheumatoid factors present a challenge in standard immunodiffusion typing.
- A novel method is presented for typing rabbit allotypes d12 and A8 by exploiting rheumatoid factor interactions.