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Leukocyte apoptosis in whole blood involves platelet-dependent coaggregation
S Hartz1, B Menart, D Tschoepe
1German Diabetes Research Institute, Heinrich-Heine-University, Duesseldorf, Germany.
Summary
Apoptotic leukocytes, particularly within platelet-leukocyte coaggregates, appear to promote platelet activation. This finding suggests apoptotic leukocytes may act as a prothrombotic trigger in inflammatory and thrombotic events.
Area of Science:
- Immunology
- Hematology
- Cell Biology
Background:
- Leukocyte activation and platelet-leukocyte interactions are implicated in thrombotic and inflammatory diseases.
- Leukocyte apoptosis is crucial for resolving inflammation.
Purpose of the Study:
- To quantify apoptotic peripheral blood leukocytes (PBLs).
- To investigate the relationship between PBL apoptosis and platelet-leukocyte interactions.
Main Methods:
- Flow cytometry using APO 2.7 antibody to detect apoptosis.
- Analysis of apoptotic PBLs in whole blood from healthy subjects.
- Induction of PBL apoptosis in vitro using phorbol 12-myristate 13-acetate.
Main Results:
- Apoptosis was detected in neutrophils, monocytes, and lymphocytes.
- Apoptotic leukocytes were predominantly found in platelet-leukocyte coaggregates.
- In vitro induced apoptosis significantly increased leukocyte-platelet coaggregation.
Conclusions:
- Apoptosis and platelet-leukocyte interactions are closely linked.
- Apoptotic leukocytes may initiate platelet adhesion and activation.
- Apoptotic leukocytes represent a potential prothrombotic factor in thrombotic events.