Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method to demonstrate normal and metaplastic Paneth cells in tissue sections.

C A Rubio1, G Nesi

  • 1Gastrointestinal and Liver Pathology Research Laboratory, Karolinska Institute and Hospital, 171 76 Stockholm, Sweden. Carlos.Rubio@onkpat.ki.se

In Vivo (Athens, Greece)
|March 27, 2003
PubMed
Summary

Autofluorescence in hematoxylin and eosin (H&E) stained sections allows for accurate Paneth cell counting. Incident-light fluorescence (ILF) improves enumeration of Paneth cells, especially in inflamed tissues.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gender and cystectomy for bladder cancer: A high-volume tertiary urologic care center experience.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2023
Same author

Carbonic anhydrases III and IX are new players in the crosstalk between adrenocortical carcinoma and its altered adipose microenvironment.

Journal of endocrinological investigation·2023
Same author

Testosterone does not affect lower urinary tract symptoms while improving markers of prostatitis in men with benign prostatic hyperplasia: a randomized clinical trial.

Journal of endocrinological investigation·2022
Same author

The IGF2 methylation score for adrenocortical cancer: an ENSAT validation study.

Endocrine-related cancer·2020
Same author

Inverted urothelial papilloma of the upper urinary tract: description of two cases with systematic literature review.

Diagnostic pathology·2020
Same author

Radiotherapy after prostatectomy in high‑risk prostate cancer.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2020

Area of Science:

  • Histopathology and Cell Biology
  • Gastroenterology and Digestive Diseases

Background:

  • Paneth cells are typically identified by eosinophilic granules in hematoxylin and eosin (H&E) stained sections.
  • Accurate enumeration of Paneth cells can be challenging, particularly in cases with cell clustering or inflammation.
  • Paneth cells exhibit autofluorescence under specific lighting conditions.

Purpose of the Study:

  • To evaluate the utility of Paneth cell autofluorescence in H&E stained sections for accurate cell counting.
  • To compare the effectiveness of incident-light fluorescence (ILF) versus transmitted light (TL) for Paneth cell enumeration.

Main Methods:

  • Surgical specimens from the small bowel (n=30) and colon with inflammatory bowel disease (IBD) (n=10) were analyzed.
  • Consecutive sections were stained with H&E and immunostained for lysozyme (Lz).

Related Experiment Videos

  • Paneth cell counts were performed in 20 crypts per case using both TL and ILF on H&E sections.

    • Main Results:

    • Incident-light fluorescence (ILF) enabled accurate assessment of Paneth cells, even in crypts with significant cell clustering.
    • Transmitted light (TL) provided less precise counts in areas with numerous or clustered Paneth cells.
    • Mean Paneth cell counts were 3.9 cells/crypt in normal small bowel and 3.3 cells/crypt in IBD colon using H&E-ILF.

    Conclusions:

    • Autofluorescence observed with ILF on H&E stained sections offers a reliable method for enumerating Paneth cells.
    • This technique enhances visualization and improves the accuracy of Paneth cell quantification in both normal and metaplastic states.
    • The H&E-ILF method is valuable for research and clinical trials requiring precise Paneth cell counts.