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Updated: Sep 4, 2026
FISH for Pre-implantation Genetic Diagnosis
Published on: February 24, 2011
[Single cell PCR for sex determination in preimplantation genetic diagnosis]
1National Laboratory of Medical Genetics, Central South University, Changsha 410078, China.
Objective:
To develop an efficient, rapid and reliable method for sex determination in preimplantation genetic diagnosis.
Methods:
Nested PCR and fluorescent PCR were used to amplify amelogenin and IL-3 genes from human single lymphocytes and blastomeres.
Results:
The amplification efficiencies of nested PCR and fluorescent PCR of man single lymphocyte/woman single lymphocyte/blastomere were 64.7%/65%/85.7%, and 72%/76%/86.9% respectively. The PCR products of the lymphocyte were exactly the same as those of the donor gDNA. The PCR products of blastomeres coming from the same embryo were all identical.
Conclusion:
The amplification efficiencies of nested PCR and fluorescent PCR are similar while fluorescent PCR is more conventional, time sparing and sensitive.
