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Updated: Sep 26, 2026
Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model
Published on: October 2, 2015
[A modified method for isolating and culturing peritoneal mesothelial cells in rats]
Min Fan1, Fu-you Liu, Shao-bin Duan
1Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Objective:
To establish a culture model of peritoneal mesothelial cells in vitro separated by infusing the digestive fluid in the rat abdominal cativity.
Methods:
Peritoneal mesothelial cells (PMC) were digested with 0.125% trypsin-0.01% EDTA Na2 in the abdominal cavity. We collected coelio-perfusate and cultured it. The isolated cells were identified by the phase contrast microscope, electron microscope and immunohistochemical analysis.
Results:
The confluent cells showed a uniform cobblestone-like appearance under the phase contrast microscope; numerous surface microvilli could be found under the electron microscope and the purity of the cells was over 95%. Immunohistochemical studies revealed a positive staining for cytokeratin and vimentin, but a negative staining for Factor VIII associated antigen and CD45.
Conclusion:
The model of rat peritoneal mesothelial cells has been established successfully and it will provide an experimental basis of studying the fibrosis in peritoneal dialysis.
Insights
Researchers successfully cultured rat peritoneal mesothelial cells (PMC) in vitro, establishing a reliable model for studying peritoneal dialysis complications like fibrosis.
Area of Science:
- Cell Biology
- In Vitro Models
Context:
- Peritoneal dialysis is a common treatment for kidney failure.
- Understanding peritoneal complications is crucial for patient outcomes.
Purpose:
- To establish a reproducible in vitro culture model of rat peritoneal mesothelial cells (PMC).
- To provide a foundation for investigating peritoneal fibrosis in the context of dialysis.
Summary:
- Peritoneal mesothelial cells were isolated from rat abdominal fluid using trypsin-EDTA digestion.
- Cell cultures exhibited characteristic morphology (cobblestone appearance, microvilli) and high purity (>95%).
- Immunohistochemistry confirmed PMC identity (cytokeratin+, vimentin+, Factor VIII-, CD45-).
Impact:
- Successful establishment of a rat PMC in vitro model.
- Facilitates experimental research into peritoneal dialysis-related fibrosis.
- Supports advancements in understanding and treating peritoneal dialysis complications.
