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Updated: Aug 2, 2026

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Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Published on: May 29, 2011
[New fluorescent bichromophoric probes for studying mechanisms of donor-donor energy migration (DDEM)]
L B Johansson1, S V Kalinin, K A Filatova
1Umeå University, Umeå, Sweden.
Bioorganicheskaia Khimiia
|March 28, 2003
Abstract:
Three fluorescent probes were synthesized for studying the excitation energy migration between two identical fluorophores. Each probe has two identical fluorescent groups (dansyl, 7-nitrobenz-2-oxa-1,3-diazole-4-yl, or fluoresceinyl) linked by the rigid bis-(8-aminooctyl)amide of 4,4'-biphenyldicarbonic acid or flexible dotriacontanedioic acid spacer, which enables the intramolecular energy migration through the distance of 3.2-3.5 nm.

