Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Amino acid clusters formed by sonic spray ionization.

Zoltan Takats1, Sergio C Nanita, R Graham Cooks

  • 1Purdue University, Department of Chemistry, West Lafayette, Indiana 47907, USA.

Analytical Chemistry
|March 28, 2003
PubMed
Summary

A new sonic spray ionization source efficiently generates amino acid clusters, offering superior signal intensity and sensitivity for mass spectrometry. This method provides insights into gas-phase ion formation and shows chiral selectivity for certain amino acids.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding Mass Spectrometry: From Ion Generation to Spectral Interpretation.

Journal of mass spectrometry : JMS·2026
Same author

Mapping Functionally Relevant Tractable Lysines of Challenging Protein Targets by Covalent Fragment Screening.

Chembiochem : a European journal of chemical biology·2026
Same author

Mapping the Substrate Specificity Landscape of PAD2 and PAD4 Enzymes.

Chembiochem : a European journal of chemical biology·2026
Same author

Characterization of blood serum and cell lines by an automated high-throughput well plate sampling system using laser ablation-rapid evaporative ionization mass spectrometry.

Analytical and bioanalytical chemistry·2026
Same author

Deterministic Acceptance Limits for Statistical Equivalence Testing in Hydrogen/Deuterium Exchange Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

Plant-derived nanovesicles from <i>Ginkgo biloba</i> seeds mitigate LPS-induced endothelial dysfunction and promote vascular homeostasis.

Frontiers in bioengineering and biotechnology·2026

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Biochemistry

Background:

  • Mass spectrometry is a powerful analytical technique.
  • Ionization methods are crucial for sample introduction and detection.
  • Sonic spray ionization (SSI) is a novel ionization technique.

Purpose of the Study:

  • To develop and optimize a novel ion source based on sonic spray ionization.
  • To investigate the formation of amino acid clusters using SSI.
  • To understand the mechanisms of gas-phase ion formation in SSI.

Main Methods:

  • Construction of a novel ion source utilizing sonic spray ionization.
  • Optimization of instrumental and sample parameters for cluster ion generation.
  • Mass spectrometric analysis of amino acid clusters, particularly serine oligomers.

Related Experiment Videos

  • Comparison of SSI performance with electrospray ionization (ESI).

    • Main Results:

    • The SSI source demonstrated an order of magnitude improvement in signal intensity and signal-to-noise ratio compared to ESI.
    • Optimized conditions yielded a limit of detection of 10 nM for protonated serine octamers with a 4-order linear dynamic range.
    • Ion formation saturation was observed above 1 mM, supporting a charge residue model for SSI.
    • Chiral preference for homochiral cluster formation was observed for threonine and cysteine substitutions.

    Conclusions:

    • The developed SSI ion source is highly efficient for generating amino acid clusters.
    • SSI provides valuable insights into gas-phase ion formation mechanisms.
    • The technique exhibits sensitivity and selectivity, with potential for chiral analysis of amino acids.