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Differential gene expression in chemically induced mouse lung adenomas.
Ruisheng Yao1, Yian Wang, Ronald A Lubet
1Division of Human Cancer Genetics and School of Public Health, The Ohio State University James Cancer Center, Columbus, OH 43210, USA.
Neoplasia (New York, N.Y.)
|March 28, 2003
Summary
This study used competitive cDNA library screening (CCLS) to identify gene expression changes in mouse lung adenomas. Researchers discovered numerous overexpressed and underexpressed genes, aiding lung cancer research.
Area of Science:
- Molecular Biology
- Genomics
- Cancer Research
Background:
- Mouse lung tumor models are crucial for evaluating lung cancer chemoprevention due to similarities with human disease.
- Understanding gene expression alterations is key to deciphering lung carcinogenesis.
Purpose of the Study:
- To identify messenger RNA (mRNA) expression changes in chemically induced lung adenomas compared to normal lung tissue using competitive cDNA library screening (CCLS).
- To discover novel genes and known genes with altered expression relevant to lung carcinogenesis.
Main Methods:
- Utilized competitive cDNA library screening (CCLS) on over 160,000 clones from a mouse lung cDNA library.
- Confirmed altered gene expression through dot blot hybridization and semiquantitative reverse transcription polymerase chain reaction (RT-PCR).
Main Results:
- Identified 2555 clones with altered mRNA expression, with 515 overexpressed and 240 underexpressed in tumors.
- Confirmed reproducible alterations in 65 clones, including known genes like ERK-1, JAK-1, and surfactant proteins, and novel genes.
- Observed significant gene expression changes in over 60% of lesions, indicating consistent molecular alterations.
Conclusions:
- Competitive cDNA library screening (CCLS) is an effective method for identifying genes with altered expression in lung adenomas.
- The identified genes provide insights into the genetic basis of lung carcinogenesis and potential biomarkers for chemoprevention studies.