Related Experiment Video

Updated: Sep 26, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

c-Myc transformation domain recruits the human STAGA complex and requires TRRAP and GCN5 acetylase activity for

Xiaohui Liu1, Jerusalem Tesfai, Yvonne A Evrard

  • 1Department of Biochemistry, University of California, Riverside 92521, USA.

The Journal of Biological Chemistry
|March 28, 2003
PubMed

Insights

The c-Myc oncoprotein (Myc) recruits the STAGA complex, including TRRAP and GCN5, to activate gene transcription in cancer. This interaction enhances cell proliferation and apoptosis control.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Epigenetics

Background:

  • Deregulation of c-Myc oncoprotein (Myc) is a key factor in numerous cancers.
  • Myc functions as a transcription factor controlling cell proliferation and apoptosis, but its mechanisms are not fully understood.
  • Myc's role in cell transformation involves interactions with TRRAP and GCN5, components of histone acetyltransferase (HAT) complexes.

Purpose of the Study:

  • To identify the specific HAT complexes recruited by Myc.
  • To elucidate the roles of TRRAP and GCN5 in Myc-mediated transcription activation.
  • To investigate the physical interactions between Myc, TRRAP, GCN5, and the STAGA complex.

Main Methods:

  • Investigated direct physical interactions between Myc's N-terminal domain and the STAGA coactivator complex.
  • Assessed the cooperative effects of GCN5 and TRRAP on Myc-induced transcription activation in vivo.
  • Determined the requirement of TRRAP's SPT3/GCN5 interaction domain and GCN5's HAT activity for synergistic transcription enhancement.

Main Results:

  • Myc directly co-recruits TRRAP and GCN5 through interactions with the human STAGA complex.
  • GCN5 and TRRAP synergistically enhance transcription activation mediated by Myc's N-terminal domain.
  • This synergistic effect is dependent on the SPT3/GCN5 interaction domain of TRRAP and the HAT activity of GCN5.

Conclusions:

  • Myc utilizes the STAGA complex, specifically TRRAP and GCN5, for transcriptional regulation.
  • TRRAP acts as an adaptor within STAGA, facilitating GCN5 HAT recruitment to Myc during transcription activation.
  • Understanding this mechanism provides insights into Myc's role in cancer and potential therapeutic targets.

More Related Videos

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...