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Identification and quantification of antigens associated with HLA class I molecules on a bladder tumour cell line.
Nasir Torabi-Pour1, W John W Morrow, Roghieh Saffie
1Uro/Oncology Research and Department of Immunology, St. Bartholomew's and The Royal London School of Medicine and Dentistry, London EC1A 7BE, UK.
Abstract:
The aim of this study was to isolate and identify antigenic peptides associated with HLA class I molecules on a bladder tumour cell line. HLA-A1 molecules were purified using an immunobead-purification technique and following elution of nonapeptides associated with the complex, their HPLC profile was determined by Tandem mass spectrometry (MS/MS). Three peptides were identified namely: (1) P991 (VTDPGNLLY); (2) P1041.5 (LTDLGFLVY), and (3) P1057.7 (LTDPHLLSY); these sequences matched elements of hepatitis B, MAGE1-A1 and herpes simplex viruses. These antigens had half-lives of approximately 120 min which is within the theoretical range of such short peptides. These results indicate that identification of MHC-associated peptides is possible without using an assay for cytotoxic T cells. Although this approach was applied on a relatively small scale, broader applications can be foreseen.