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Technical note: Specific PCR amplification of protozoal 18S rDNA sequences from DNA extracted from ruminal samples of

S K R Karnati1, Z Yu, J T Sylvester

  • 1Department of Animal Sciences, The Ohio State University, Columbus 43210, USA.

Journal of Animal Science
|March 29, 2003
PubMed

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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...