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Apoptotic activity in stored human platelets.

Peter L Perrotta1, Cheryl L Perrotta, Edward L Snyder

  • 1Department of Pathology, State University of New York at Stony Brook, Nicolls Road, Stony Brook, NY 11794-7300, USA. pperrotta@notes.cc.sunysb.edu

Transfusion
|March 29, 2003
PubMed
Summary
This summary is machine-generated.

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Platelet mitochondria maintain their potential during storage. However, storage and stress increase markers of platelet death, indicating mitochondrial potential changes do not predict apoptosis in platelets.

Area of Science:

  • Hematology
  • Cell Biology
  • Mitochondrial Research

Background:

  • Platelets have apoptotic machinery, but mitochondrial function during cell death is understudied.
  • Mitochondrial viability is crucial in cell death pathways and requires investigation in platelets.

Purpose of the Study:

  • To evaluate mitochondrial viability in stored and stressed platelets.
  • To assess apoptotic markers in relation to mitochondrial function.

Main Methods:

  • Platelet mitochondrial transmembrane potentials (Deltapsim) measured using JC-1 dye.
  • Annexin V binding, CD62P expression, and caspase-3 activity assessed via flow cytometry and enzyme assays.
  • Platelets subjected to storage, azide, calcium ionophore, and plasma deprivation.

Related Experiment Videos

Main Results:

  • Deltapsim remained stable in platelets stored for 7 days.
  • Stored platelets showed increased phosphatidylserine (PS) exposure, CD62P expression, and caspase-3 activity.
  • Stressed platelets exhibited altered Deltapsim, PS exposure, and CD62P expression.

Conclusions:

  • Platelet mitochondria maintain Deltapsim during standard 7-day storage.
  • Changes in Deltapsim do not correlate with downstream apoptotic markers like caspase activation and PS exposure in stored platelets.