Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human gastrointestinal sulfotransferases: identification and distribution.

Guangping Chen1, Daqing Zhang, Nin Jing

  • 1Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA. cguang@okstate.edu

Toxicology and Applied Pharmacology
|March 29, 2003
PubMed
Summary

Sulfotransferases (STs) in the human gastrointestinal tract show varied activity. Small intestine STs exhibit higher sulfation activity for key substrates compared to the stomach and colon.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural Characterization and Expression Profiling of Ethylene Biosynthetic Genes During AgNO<sub>3</sub>-Induced Sex Reversal in Bitter Gourd.

International journal of molecular sciences·2026
Same author

Exploring the feasibility of modeling next-day fatigue and sleepiness using digital sleep tracker data in neurodegenerative and immune-mediated inflammatory diseases.

Frontiers in digital health·2026
Same author

Research on trajectory tracking control of tracked vehicles based on hydraulic motor system identification and Laguerre-MPC.

PloS one·2026
Same author

The Chinese Medical Journal and the Chinese Medical Association: Centennial legacy and the institutionalization of a scholarly community.

Chinese medical journal·2026
Same author

Socioeconomic inequalities in online fresh food delivery use and associations with the neighborhood food environment.

BMC public health·2026
Same author

Hypertriglyceridaemia and its multifaceted mechanisms driving atherosclerotic cardiovascular disease​​.

Lipids in health and disease·2026

Area of Science:

  • Biochemistry
  • Pharmacology
  • Gastroenterology

Background:

  • Sulfotransferases (STs) are crucial enzymes catalyzing sulfation reactions for diverse molecules.
  • Understanding ST distribution and activity in the human gastrointestinal tract is vital for drug metabolism and xenobiotic processing.

Purpose of the Study:

  • To characterize sulfotransferase activities in various segments of the human gastrointestinal tract.
  • To compare ST activity in the intestine with that of the liver.
  • To investigate donor-to-donor variability in intestinal ST activity.

Main Methods:

  • Enzymatic assays were performed using substrates like 2-naphthol, dopamine, estradiol, and dehydroepiandrosterone (DHEA).
  • Cytosol from stomach, duodenum, small intestine, and colon from 23 human donors was analyzed.

Related Experiment Videos

  • Western blot analysis was used to confirm enzymatic assay findings.

    • Main Results:

    • The small intestine exhibited higher sulfation activity for all tested substrates compared to the stomach and colon.
    • Small intestine 2-naphthol sulfation activity was approximately half of liver activity, while dopamine sulfation was threefold higher.
    • Significant donor-to-donor variation in ST activity was observed along the length of the small intestine.

    Conclusions:

    • Human small intestinal STs play a significant role in sulfation, with activity levels varying considerably among individuals.
    • The observed variability suggests potential regulation of intestinal STs by xenobiotics.
    • These findings have implications for understanding drug metabolism and the disposition of endogenous compounds in the gastrointestinal tract.