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Related Experiment Videos

Changes in rhinovirus protein 2C allow efficient replication in mouse cells.

Julie R Harris1, Vincent R Racaniello

  • 1Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.

Journal of Virology
|March 29, 2003
PubMed
Summary
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Researchers adapted rhinovirus type 16 to infect mouse cells efficiently. This new variant, 16/L, shows increased viral replication and cytopathic effects, aiding in developing a mouse model for rhinovirus infection.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Rhinovirus type 16 replicates in mouse L cells expressing human intercellular adhesion molecule 1 (ICAM-1).
  • Low multiplicity infections yield minimal cytopathic effect and low virus titers.
  • Efficient viral replication is crucial for studying rhinovirus pathogenesis.

Purpose of the Study:

  • To characterize a rhinovirus host range mutant.
  • To investigate cellular proteins essential for efficient viral growth.
  • To develop a murine model for rhinovirus infection.

Main Methods:

  • Isolation of a rhinovirus 16 variant (16/L) through alternate passage in HeLa and ICAM-1 L cells.
  • Analysis of viral replication, RNA production, and cytopathic effects in infected mouse cells.

Related Experiment Videos

  • Identification of genetic mutations in the P2 region and protein 2C of the 16/L variant.

    • Main Results:

    • The 16/L variant demonstrated enhanced replication in mouse cells, leading to higher virus titers.
    • Increased viral RNA production and complete cytopathic effect were observed with the 16/L variant.
    • Three amino acid changes were identified in the P2 region, with two changes in protein 2C linked to the adaptation phenotype.

    Conclusions:

    • The characterization of the 16/L rhinovirus mutant provides insights into host-pathogen interactions.
    • This adapted virus facilitates the study of cellular factors governing rhinovirus replication.
    • The development of a rhinovirus murine model is a significant step towards understanding infection and developing therapies.