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A simple DNA extraction method for marijuana samples used in amplified fragment length polymorphism (AFLP) analysis
Heather Miller Coyle1, Gary Shutler, Sharon Abrams
1Division of Scientific Services, Department of Public Safety, 278 Colony Street, Meriden, CT 06451, USA. C4ensic@yahoo.com
Abstract:
As a first step in developing a molecular method for the individualization of marijuana samples, we evaluated a plant DNA extraction kit. The QIAGEN plant DNeasy method uses a spin column format for recovery of DNA and is effective for obtaining high molecular weight DNA from leaf, flower (bud), and seed samples of marijuana. The average DNA yield was 125-500 ng per 100 milligrams of fresh plant tissue. The recovered DNA was of polymerase chain reaction (PCR) quality as measured by the ability to generate reproducible amplified fragment length polymorphism (AFLP) profiles. AFLP is a technique used to create a DNA profile for plant varieties and is being applied to marijuana samples by the authors to link growers and distributors of clonal material. The QIAGEN plant DNeasy method was simple, efficient, and reproducible for processing small quantities of marijuana into DNA.
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