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Related Experiment Videos

Expression and regulation of eHAND during limb development.

Marian Fernandez-Teran1, M Elisa Piedra, Jose C Rodriguez-Rey

  • 1Departamento de Anatomía y Biología Celular, Universidad de Cantabria, Spain.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|April 1, 2003
PubMed
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The eHAND transcription factor shows dynamic expression during limb development, primarily in ventral and anterior mesoderm. Its expression is regulated by signaling pathways and is crucial for establishing anteroventral limb identity.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • The bHLH transcription factor eHAND is essential for embryogenesis.
  • Understanding eHAND's role in limb development is key to deciphering limb patterning.

Purpose of the Study:

  • To investigate the dynamic expression pattern of eHAND during vertebrate limb development.
  • To explore the regulatory mechanisms and functional significance of eHAND in limb patterning.

Main Methods:

  • Expression analysis in chick and mouse embryos.
  • Analysis of mutant limbs (talpid(2), oligozeugodactyly).
  • Manipulation of signaling pathways (SHH, retinoic acid, BMP).
  • Overexpression studies using retroviral vectors.

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Main Results:

  • eHAND exhibits dynamic expression in ventral and anterior mesoderm during limb development, with species-specific differences.
  • eHAND expression is down-regulated by anterior mesoderm respecification and absent in posteriorized limbs.
  • Dorsal ectoderm represses eHAND, while BMP signaling positively regulates it.
  • Overexpression of eHAND leads to minor phenotypic alterations like digit duplication.

Conclusions:

  • eHAND expression is linked to the anteroventral identity of limb mesoderm.
  • eHAND plays a role in limb patterning, influenced by signaling gradients and ectodermal-mesenchymal interactions.
  • Further research is needed to fully elucidate eHAND's function and downstream targets in limb development.