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Updated: Sep 26, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Circular dichroism and the interactions of water soluble porphyrins with DNA
1Department of Chemistry, Swarthmore College, Swarthmore, Pennsylvania 19081, USA. rpaster1@swarthmore.edu
Abstract:
The size, sign, and profile of induced circular dichroism (CD) features in the Soret region are reliable indicators of the binding modes of porphyrins and metalloporphyrins to DNA. Porphyrins shown (using such CD criteria) to be intercalators in monodispersed DNA duplexes prove extremely useful for the detection and characterization of organized, condensed forms of nucleic acids (psi-condensates). In addition, certain select porphyrin derivatives can form extended assemblies on nonaggregated DNA templates. A combination of CD and resonance light scattering (RLS) measurements allows for sensitive detection and characterization of these porphyrin arrays.
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