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Updated: Sep 26, 2026
On-Chip Endothelial Inflammatory Phenotyping
Published on: July 21, 2012
Homocysteine alters monocyte-endothelial interaction in vitro
Xuewei Guo1, Nicholas Peter Dudman
1Center of Cardiovascular Disease Research, Gansu Province People's Hospital, Lanzhou 730000, China. Xueweiguo@163.net
Objective:
To determine whether homocysteine induced endothelial damage through monocyte-endothelial interaction and to characterize both cell types in vitro.
Methods:
Radiomethods were performed on monocyte adhesion to/through endothelium and endothelial damage experiments.
Results:
Homocysteine-treated endothelial cells increased monocyte adhesion and transmigration. Homocysteine-treated monocytes induced endothelial detachment, but this effect was blocked by catalase. These effects were increased with higher concentrations of homocysteine. Monocyte surface glycoprotein antibodies CD11b/CD18 and CD14 inhibited these processes.
Conclusions:
Homocysteine alters monocyte-endothelial interaction in vitro, eventually bringing about endothelial damage through release of H(2)O(2). These phenomena are mediated through monocyte surface glycoproteins CD11b/CD18 and CD14. Upregulation of these processes in vivo may contribute to acceleration of atherosclerosis in patients with elevated plasma homocysteine levels.
